A better way to isolate exosomes
"We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1."
Need exosomes? SBI's ExoQuick-TC enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 1 ml) of tissue culture media and certain biofluids (saliva, urine, follicular fluid, and breast milk). Note, to isolate exosomes from serum, plasma, or ascites fluid, use the original ExoQuick formulation (EXOQ5A-1 or EXOQ20A-1). Compatible with a wide variety of downstream applications, ExoQuick-TC is an effective and proven alternative to ultracentrifugation1-3.
ExoQuick-TC’s fast, ultracentrifugation-free method:
Saves time and labor
Is easily scalable
Conserves precious sample
Delivers high yields of functional, high quality exosomes
Can be used to isolate exosomes for a wide range of downstream applications, including
Biomarker studies
Exosomal miRNA profiling
Exosomal proteomics
Exosomal lipidomics/metabolomics
Functional studies, such as in cell-to-cell signaling
Basic biology, such as role in tumorigenesis
ExoQuick-TC is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick-TC to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.